Integrated Molecular Technologies Core (IMTC) and Microbiome Sub-Core

The overarching objective of the Integrated Molecular Technologies (IMTC) Core is to promote and facilitate basic and translational GI research by providing UCLA: DDRC investigators with access to state-of-the-art molecular technologies, including: 1) expertise, equipment and services for systems biology and high-throughput screening analyses; 2) expertise and viral vector cloning and production services to genetically engineer mammalian cell lines and primary cells for expression or inhibition of proteins and miRNAs both in vitro and in vivo. 3) expertise, equipment, and services to perform multi'omics microbiome studies.

 The IMT Core, a merger of the former Vector Core and the Systems Biomedicine Center, represents an integrated unit intended to maximize efficiency and ease of utilization of molecular services by providing UCLA: DDRCC investigators with access to state-of-the-art molecular technologies that enable efficient gene transfer, protein expression, and functional analysis in mammalian cells in culture and in vivo, as well as to perform protein analyses, genomic profiling, cellular phenotyping and high-throughput molecular screening. These technologies will assist UCLA: DDRCC investigators in their research efforts to understand the functional significance of specific genes and proteins during normal development and organogenesis in the gastrointestinal tract and to determine and answer fundamental  questions on the  functional significance of specific candidates genes and proteins to the pathogenesis or amelioration of digestive diseases, and to identify and validate novel therapeutic targets for conventional and experimental therapies. 

 The IMTC services include (but are not limited to):  (1) consultation and troubleshooting in techniques for efficient gene delivery, as well as cloning  and functional protein expression in mammalian cells in vitro and in vivo, (2) consultation and to perform customized gene expression profiling, cytological phenotype profiling, and high-throughput molecular screening studies by utilizing high-throughput and highly automated robotic systems. The Integrated Molecular Technologies Core (IMTC)  is therefore intended to serve as a comprehensive resource that will provide access to state-of-the-art molecular tools and thereby aims to facilitate the research activities of UCLA: DDRCC investigators.  3) consultation on microbiome study design an da compreshensive suite of sequencing-based series for characterizing mircobiome composition and function.

Technical Services:

 Gene Transfer: Cloning , Retroviral vectors, Lentiviral vectors, Adenoviral vectors, and  AAV

 High-Throughput Molecular Profiling Platforms:  The Nanostring system can examine the expression levels of up to 800 genes or 750 microRNAs in the same sample in ~6 hr.

Luminex FlexMap3D system (BioRad) is available for high-throughput cytokine/chemokine and phosphoproteomic analyses, and can examine 96 samples simultaneously.   BioRad Bio-Plex 3D with a Suspension Array System and Bio-Plex Pro II Wash Station is available for processing of xMAP assays which can be built on either magnetic beads or non-magnetic beads.

Drug Discovery Platforms: Biomek FX and NX automated workstation equipped with a robotic transport system (Beckman-Coulter) and EnVision multilabel plate reader (PerkinElmer) is available for high-throughput molecular screening studies to identify novel therapeutic candidates. More than 100,000 chemical compounds are available for high-throughput chemical screening on this platform.

Cellular Modeling Platform:  The xCELLigence system (Roche Applied Science) is available for monitoring of cellular events in real time without the need for incorporation of labels, and can be used to quantitate cell number

IMTC Contact Information:

Emmanuelle Faure, PhD
Lab Manager
(310) 825-7112

Microbiome Sub- Core Services Contact:

Jonathan Jacobs, MD, PhD
(310) 825-9333

Venu Lagishetty, PhD